Description:
Portable gene gun is a handy handheld device for rapid, direct in situ gene transfer of cells. The system uses adjustable helium pressure to drive gold particles pre-coated with DNA, RNA or other biomaterials on the inner wall of a small plastic tube and direct it into the cell to make the transgene work more flexible Easy.
Features
- portable, can be flexible in vitro transgenic experiments
- low noise, high repeatability, easy to live animal transformation
- other methods can not be completed, there are special needs of cell growth transformation
- small shooting area, uniform particle distribution, can transform different size receptors, to ensure the conversion efficiency
- simple, fast, flexible function, a variety of cell types common
- to help transient expression and stable expression
- only a small amount of DNA and cells, without vector DNA
- to be able to carry out multiple plasmid co-introduction of large fragments of DNA unlimited
Application areas
- Animals: Any tissue (skin, organs), cells, organs etc exposed to the muzzle of the gene
- plants: plant and greenhouse plant explants
- yeast, bacteria, other microorganisms
- gene expression research
- agricultural biotechnology and crop improvement
- plant vaccine
- organelles, chloroplasts, mitochondria
Patent No .: ZL01 105513.8 200630105826.0
Technical parameters
| Factor |
GG-500 Portable Gene Gun |
|
Experimental conditions |
In situ, in vitro, in vivo, from the body |
|
Target region |
（small）2cm2 |
|
Pressure range |
0.5-5MPa |
|
Target type |
Animals: any organization (skin,organs) exposure to gene gun；cells, explant and organ culture |
|
Voltage |
9V( capable of firing 1000 times) |
|
Gas |
Helium |
|
Metal particle |
Φ0.6-1.6μm（ common Φ1.6 μm） |
System standard configuration:
Gene gun, a special gas valve and hose, loading device, cutting machine, magazine, imported nylon tube 50Feet2 package, 2 gold powder.