Description:
The gene gun technology (Particle Bombardment) is a new gene transfer technology that accelerates micron-sized gold or tungsten particles coated with nucleic acids to the desired rate to introduce genes into cells, tissues or organs. Get instantaneous expression of stability and high efficiency, transformation. With fast, easy, safe and efficient features.
Performance characteristics
- simple, fast, flexible function, a variety of cell types common
- contribute to transient expression and stable expression
- only a small amount of DNA and cells, without vector DNA
- to be able to carry out multiple plasmid co-introduction of large fragments of DNA unlimited
- in vivo and in vitro transformation can be high specificity
- bombardment room unique circular conversion room, removable, easy to clean and disinfect
- low cost of supplies, each gun except high-pressure gas costs about 18 yuan
- high repeatability
- other methods can not be completed, there are special needs of cell growth transformation
- the particles are evenly distributed
- the maximum control of the experimental process
Application areas
- animals: tissue (skin, organs), cells, organs and so on
- plants: living plants, leaves or other plant tissues and organs
- yeast, bacteria, other microorganisms
- gene expression research
- agricultural biotechnology and crop improvement
- plant vaccine
Patent No .: ZL011 05512.X200630105830.7
Technical parameters
Experimental conditions | In vitro, from the body, plants (in vivo) |
Target region | (big)40cm2 |
Pressure range | 3-12MPa |
Target Type | Animals: cell and organ culture Plants: The smaller of the whole plants, cell culture, explant, yeast, bacteria and other micro-organelles, chloroplast, mitochondria |
Voltage | 220V/110V |
Gas | Helium or nitrogen ( helium is more otimized) |
Metal Particle | Φ0.6-1.6μm,(Common Φ1μm) |
Standard configuration:
- Host
- vacuum pump
- 1000 gun supplies (3.5 / 4.5 / 7Mpa rupturable membrane, carrier membrane, stainless steel mat net, 2 bottles of powder, tungsten powder 1 bottle);