Automatic Stainless Steel Fermenter, BIOF-SA

New BIOF-SA Automatic Stainless Steel Fermenter tanks meet your pilot scale-up and small-scale production needs. Different tank capacity options from 10L to 2000L can flexibly cope with various small production scales. The tank body adopts products from domestic specially certified manufacturers to ensure reliable quality.

BIOF-SA Automatic Stainless Steel Fermenter tanks have a series of powerful functions to make users’ operations more convenient and efficient. Standard automatic sterilization technology ensures the hygiene and safety of the fermentation process. It comes standard with automatic tank pressure and tank lid lifting functions, eliminating tedious manual operations and further improving production efficiency. At the same time, the integrated tank bottom valve design eliminates the possibility of bacterial contamination in the user’s materials, making it safer and sterile, ensuring stable product quality.

In addition, BIOF-SA Automatic Stainless Steel Fermenter tanks also support personalized customization to meet specific production requirements. Whether it is an upgrade to pharmaceutical specifications or an expansion of online testing capabilities, Bioreactek™ can provide tailor-made solutions. When you choose the BIOF-SA series, you choose excellent quality and efficient production. Let BIOF-SA series stainless steel fermentation tanks be the booster for your success!

PRODUCT FEATURES

		BIOF Feed-Tec™ feeding system Customized manual and automatic feeding on time can be cascaded forward and reverse with pH and DO to accurately control parameters Configure equation feeding and index feeding to fit the bacterial growth process and meet high-density fermentation and culture control.
BIOF Easy-Tri™ Audit Trail Exclusive research and development system automatically stores all extraction records, and the experimental process can be tracked Real-time protection of experimental data, advanced permissions to restrict export and deletion, prevent data leakage, and improve system security HMI supports independent audit trails and unlimited intelligent sequence control functions
		Modular design Each group of tanks is designed with independent modules and can be freely matched with multi-connection systems. Support multi-tank system and amplified tank system Modular design does not require changes in pipeline and layout design, flexible combination, and no duplication and waste in later changes and modifications
One-click operating system One-click fermentation and one-click sterilization Custom parameter configuration With power outage recovery function, data is protected during power outage. Supports three-level authority management, making experimental data safer Can expand the host computer system and IoT remote system

Fermenter Specifications

MODEL

BIOF-S10A

BIOF-S30A

BIOF-S50A

BIOF-S 100A

BIOF-S200A

BIOF-S300A

BIOF-S500A

BIOF-S1000A

BIOF-S2000A

Stainless steel tank

Tank size (L)

100

200

300

500

1000

2000

Working volume(L)

3-8

8-24

12-40

25-80

40-160

80-240

100-400

200-800

400-1600

Inner tank material

AISI 316L/AISI 304L stainless steel

Jacket material

AISI 304L stainless steel

Manufacturing Standards

ASME BPE-2019, PED welding standards

Polishing degree

Polishing accuracy of surface in contact with material Ras0.4

Stirring system

Stirring power

Panasonic servo motor/SEW reduction motor; PID control

Stirring speed

20~800rpm ±1rpm

20~600rpm

±1rpm

20~400rpm

+1rpm

20~300rpm

±1rpm

impeller

Inclined blade type/spiral type/paddle type/turbine type/propulsion type, etc.

Temperature control

Temperature control method

PT100; PID control; temperature adjustment is completed under the action of plate heat exchanger, electric heating jacket and cooling water

Heating form

Temperature control jacket

Temperature control range

5℃~60℃ above ambient temperature; display accuracy 0.1℃, control accuracy ±0.1℃

Detection probe

METTLER TOLEDO/Hamilton

0~12, PID control, accuracy ±0.02

Completed under the control of acid/alkali peristaltic pump or CO₂ mass flow meter

METTLER TOLEDO/Hamilton 0~200%, PID control, accuracy ±1%

Air supply system

flow regulation

Imported glass rotor flowmeter, Air supports additional CO₂/O₂/N₂ and multi-channel gas ventilation strategies, and supports upgrade to MFC thermal mass flow meter

gas distributor

Annular distributor/microporous diffuser

filter

0.2μm coarse filtration, 0.01μm fine filtration, polytetrafluoroethylene;

Exhaust gas filter housing with heating blanket (optional)

Exhaust gas condenser

AISI 316L/AISI 304L stainless steel

Feeding system (can be customized)

peristaltic pump

Standard configuration: 4 high-precision flip-top peristaltic pumps; PID interlock control, feeding, acid, alkali and defoaming pumps; additional feeding pumps can be added

Interface (Customizable)

interface

Material liquid and gas inlet and outlet: inoculation valve interface, feeding valve interface (including off-position probe, four-valve group, etc.), online sampling port, bottom valve, surface vent, deep vent, safety bursting disc/pressure relief valve ;

Running status visualization window and searchlight

Monitoring probe interface: pH /DO / liquid level / pressure / foam, etc.

Key component brands of Automatic Stainless Steel Fermenter

PART NAME	BRAND
pH sensor	METTLER TOLEDO / Hamilton
DO sensor	METTLER TOLEDO / Hamilton
pH/DO signal line	METTLER TOLEDO /Hamilton
controller	German Siemens SIMATIC S7-1200 original series/PID program control
touchscreen	HMI TPC10
Machinery Seal	Burgmann
peristaltic pump	Bioreactek/Watson-Marlow
Feed bottle	Germany SCHOTT
Glass rotor flow meter	United States Dwyer
PT100 sensor	German JUMO
Low voltage electrical	Schneider
Manual/automatic diaphragm valve	Hebron/Gemü/Bürkert
Circulating pump	Denmark GRUNDFOS
Mass flowmeter	Switzerland Vögtlin
Stirring motor	Panasonic Servo/SEW geared motor
The electromagnetic valve	Airtac Airtac /SMC
trap	Spirax sarco/TLV
Tank pressure sensor tank pressure regulating valve	German BDISENSORS

Configuration and function expansion

Item	sketch	Description	Config
Sterilization method		Automatic online high-pressure steam sterilization The complete system adopts a complete automatic sterilization system to reduce user on-site operations and possible safety issues. Steam valves use angle seat valves and diaphragm valves The steam trap adopts imported trap Sterilization design temperature: 100~145C Sterilization 4-way steam: jacket, tank, valve, filter	·
Can lid lifting		Automatic can lid lifting device Electric linear actuator push Stroke: 400mm, guide rail guide, stable stroke All lifting interference pipelines are protected and avoided in advance to avoid damage caused by lifting On-site PLC communication, HMI display and operation Alarm value can be set	·
Tank pressure control		Automatic tank pressure The tank pressure uses BDISENSORS sensor to communicate with the host PLC, and Burkert proportional valve is automatically controlled by PID. Tank pressure automatic control range 0~0.2MPa Control accuracy ±0.001Mpa	·
Valve category		Integrated tank bottom valve Hebron / Gemu / Burkert Simple structure, easy to use, good sealing, eliminating dead spots of bacterial contamination at the bottom of traditional tanks, online steam sterilization with one-button system, automatic control, and improved work efficiency	·
Cleaning method		Automatically controlled cleaning (requires CIP cleaning system) The entire CIP system adopts a closed-circuit circulation and no dead-end cleaning design Tanks, pipes, valves, etc. do not need to be disassembled and can be cleaned in situ. Automatic temperature control, automatic remote control of water replenishment	·
multi-tank system		Multi-tank expansion Support tanks of different volumes to form a joint system to support the construction of grade tank modules Support the construction of multi-tank system BIOF-Control V2.2 control system allows one-click control of multiple tanks	○
Weighing system		Weighing module Supports the expansion of replenishing weighing and tank weighing modules. The tank weighing module is placed at the foot of the tank. Using professional weighing sensors and installation modules, rapid installation, monitoring and maintenance can be achieved Alarm values can be set, supporting customized linkage with the system Class model: C3, protection level: IP68 Feed weighing: OHAUS/METTLER TOLEDO	○
Extension modules		Online test ORP electrode: METTLER TOLEDO original imported; LF-K integrated transmitter, including filtering and self-correction, RS-485 communication; BIOF-M controller control and settings Foam electrode: Reduce dead corners of the tank and risk of bacterial contamination Living cell concentration: ABER-Biomass original import: can realize online detection of the concentration, density, dry weight, biomass volume, capacitance and conductivity of living cells Raman: Optimizing control processes, thereby reducing time between batches, controlling contamination risks, and increasing yields CO2 electrode: real-time online detection of CO2 percentage concentration in exhaust gas and simultaneous calculation of respiratory metabolism parameters CER, OUR and RQ Online OD: METTLER TOLEDO/OPTEK original imported; enables continuous turbidity measurement to help achieve process control in crystallization, phase separation, biomass growth (cell density) and other critical applications Exhaust gas analysis: BlueSens imported; can display CER, OUR and RQ online and other functional modules	○