Cell fusion instrument/Syncretize Electric

Description:
The GIC-3B cell fusion instrument is suitable for cell hybridization and cell fusion. It can directly observe the cell string formation and fusion phenomenon under an inverted microscope and can be used in the research of microbiology, animal medicine and biological engineering. Compared with the traditional chemical fusion method, it has the characteristics of non-toxicity to cells, high fusion efficiency, convenience and quickness. Can choose a variety of flat electrodes and needle electrodes, nickel wire electrode.

The system uses high-performance 32-bit ARM master chip, all digital design, LCD touch screen, beautiful appearance, easy and reliable operation, is the ideal tool for universities and research institutions for cell fusion teaching and research.

Cell fusion instrument principle: This machine mainly produces AC (string) pulse voltage, DC (fusion) single pulse voltage. Protoplasts are polarized and rapidly beaded by high-frequency AC pulses (30 kHz-3000 kHz48vp-p) generated by the AC (series) pulse signal generation circuit. DC (fusion) single-pulse generation circuit can be set the number of times the issue of pulse width adjustable DC high-voltage pulses (5US-5000US, 0-700V) so that the fusion electrode between the electric field strength instantaneous up to 6000V / CM.

Cell electrofusion is the use of a certain value of the frequency, voltage AC electric field, so that at a certain interval, parallel between the two protoplasts arranged in beaded. Re-use of a certain value of the HVDC pulsed electric field caused by reversible breakdown of the cell membrane, thereby inducing the mutual contact of cells fusion occurs.

Because of the applied electric field AC pulse can make the cells polarized to form dipoles, the adjacent cells due to the electrostatic attraction will be close to each other, and due to the non-uniform electric field generated between the electrodes in the fusion chamber, the cells in turn to the electric field strength The high electrode moves, arranged in a beaded shape. This arrangement is reversible. After the electric field disappears, the cells will recover to a random distribution. In the non-uniform electric field, the pressure on the cell membrane is unevenly distributed, resulting in the appearance of the plasma membrane Local reversible breakdown, the formation of micropores. It will automatically close after the electric field disappears, but irreversible breakdown will occur if the electric field strength exceeds the critical value.

Features

1. multi-function: both cell electrofusion and electroporation function
2. flexibility: with a wide range of voltage and pulse time
3. fast and efficient: simple and rapid completion of cell alignment, fusion, fusion process, only a few seconds
4. the new LCD touch operation
5. parameter setting is concise and convenient

Application areas

1. cell fusion (fusion process can be observed under an inverted microscope)
2. nuclear transfer
3. embryo operation
4. hybridoma generation
5. plant protoplast fusion
6. embryo egg gene transfer
7. live gene / drug introduction

Specifications: