Classic Automatic Stainless Steel Fermenter Bioreactor

Item name

Specifications

Model

BIOF-S

Capacity

10 ~ 20000 liters

Tank

Sterilization in place, design pressure 0.3 Mpa;

Body material: 316L stainless steel, jacket 304 stainless steel;

With special sampling valve for fermentation tank, bottom discharge valve, with side Large-view mirror, 12V safety Philips lighting, with Temperature, pH, DO, defoaming port, acid and alkali replenishment port, inoculation port, feed port, air inlet, exhaust port, seed transfer port, liquid level electrode, defoaming electrode, etc.;

Internal polishing accuracy Ra0.4, external polishing accuracy is Ra0.6, and all welds are firm, neat and beautiful.

Mixing system

Servo motor, speed governor; continuous and stable, adjustable; control accuracy ±1%

Top-type mechanical stirring system, adopts special 316L stainless steel for stirring shaft, AD mechanical seal (a special mechanical seal system that can meet higher fermentation requirements);

Adopts adjustable 2-stage 6-inclined blade stirring paddle, 1-stage compression type high-efficiency Bubble paddle, 4-level detachable baffle;

Intake & filtration

Deep ventilation is adopted, with the maximum ventilation 1.5VVM;

Primary filtration, 0.01μm precision sterilization filter, fine filter selection of high-quality products, to ensure safety and independent sterilization, greatly extending the service life; annular gas distribution at the bottom Device

pH control

Electrode: Mettler high temperature resistant glass electrode

Control range: 2-12pH, display range: 0-14pH,

Control accuracy: ±0.02pH, resolution: 0.01pH;

Control: Intelligent PID control, abnormal pH value alarm, segment control, data can be uploaded to the host computer

DO2 control

Electrode: Mettler high temperature resistant glass electrode

Control range: 0-100%, display range: 0-200%,

Control accuracy: ±5%, resolution: 0.1%;

Control: Intelligent PID control, abnormal DO value alarm, data can be uploaded to the host computer

Temperature Control System

Electric heating, water cooling, circulating pump temperature control, air escape function and water cut, over-temperature protection function, with good heat exchange efficiency;

Intelligent PID control not only ensures the rapidity of temperature control, but also realizes energy saving.

Temperature control: cooling water temperature +5℃-65℃±0.2℃, display temperature: 0-150℃±0.1℃ Fermentation process sequence control (set according to fermentation time zone, temperature automatic variable control, at least 10 sections)

Defoaming system

Antifoaming agent: flow addition by peristaltic pump

Highly sensitive defoaming electrode

Control method: time and proportion automatic control

Gas flow detection system

Air intake method: bottom annular gas distributor (enlarged aperture to effectively prevent foreign matter obstruction), controlled air intake system

Flow control: rotameter, Siemens module, MFC auto flow control is optional

Air filtration: pre-main pre-filter, secondary air inlet filter (the filter can be sterilized independently).

Fine filtration, the filter shell is made of 304 stainless steel, the filter element filtration effect can reach the sterile filtration level, the filter element material is hydrophobic polytetrafluoroethylene or other suitable materials, the sterilization filter has an online integrity test port, and each sterilization filter has an independent Online sterilization process.

Pre-filter, filter housing is made of 304 stainless steel.

Intake control: rotameter. The air intake pipeline has a diaphragm valve that can be manually opened and closed.

Check device: There is a check device to prevent the valve from being sucked back by materials.

Pressure display: displays the pressure of ventilation and sterilization process with an accuracy of 0.01MPa

Drainage: The filter is designed with a drainage device and has measures to prevent back suction.

Exhaust: The exhaust is manually controlled by a pneumatic diaphragm valve; the gas is filtered and discharged, and the filter shell is made of 304 stainless steel.

Sterilization requirements: Filters, inlet and exhaust pipelines can be steam sterilized online

Ventilation: Rotameter control, adjust and control the flow of each gas according to process requirements, the maximum ventilation flow is 2VVM. The air system consists of pre-filter, flow meter, air fine filter, diaphragm valve, and check valve.

Steam filtration precision: 1um.

* MFC Auto flow control

Vogtlin mass flow controller: The mass flow controller is used to accurately control the air intake flow, and the air flow can be automatically adjusted according to the dissolved oxygen value.

Maximum design flow is about 2VVM, and the control accuracy is ±0.5%

Pressure detection system

Direct-mounted pressure gauge

Control method: Auto control

Pressure gauge auxiliary display, control range: 0-0.4MPa, high temperature resistant and high-precision pressure sensor is used to accurately detect the fermentation tank pressure, the regulating valve automatically adjusts the tank pressure. Pressure measurement range: 0-0.35Mpa, ±0.3%, control accuracy :±0.5%

Automatic feeding system

PID automatic setting control fermentation process segmented control (according to fermentation time, automatic variable control of feeding, at least 10 segments) feeding amount curve analysis adding amount cumulative display record data storage

Equipped with a complete replenishment system, the replenishment can be measured;

Number of peristaltic pumps can be selected according to needs, and conventional configuration is four.

The role of the peristaltic pump can be selected and set on the control interface, which makes the user more convenient and flexible in actual use.

Equipped with specially designed replenishment pins to ensure the safe operation of replenishment.

SIP system

One-click fully automatic sterilization, including filters, tanks and pipelines. Sampling valve and discharge valve manual sterilization

Sterilization temperature: 100-130℃;

* Can lid lifting

Stainless steel guide rail, stepper motor

* Tank weighing system

The weighing module is used to accurately measure the fermentation liquid with an accuracy of up to 0.1%

Transplant

Can be transferred to the next level, using stainless steel valves and polished pipes;

The rationality of the design ensures the safe realization of the transfer

System equipped with valve pipeline

Valve material: AISI 316L, the valve in contact with the material adopts imported diaphragm valve

The pipeline is made of stainless steel with precision polished; argon gas shielded welding; meets GMP requirements

The stainless steel table frame is used, and the bottom is installed with pulleys for easy movement

* Automatic CIP System

lye tank

hot water tank

Diaphragm pump

CIP Outlet Pump

CIP cleaning return pump

liquid level sensor

Temperature Sensor

conductivity meter

Manual butterfly valve

Pneumatic Butterfly Valve

Control cabinet

Part Name

Brand

Origin

Remark

pH electrode

Hamilton/Mettler

Switzerland

Standard fitted

DO electrode

Hamilton/Mettler

Switzerland

Standard fitted

pH signal transmission line

Hamilton/Mettler

Switzerland

Standard fitted

DO signal transmission line

Hamilton/Mettler

Switzerland

Standard fitted

touch screen

MCGS

Well-known domestic brands

Standard fitted

PLC

Siemens

Germany

Standard fitted

Thermostatic water pump

WILO

German brand

Standard fitted

Electric machine

SEW

Germany

Standard fitted

Frequency converter

BOSCH

Germany

Standard fitted

controlling software

Bioreactek™

China

Standard fitted

The electromagnetic valve

Sanlixin/Julang

Zhejiang/Shanghai

Standard fitted

Pneumatic diaphragm valve, pneumatic angle seat valve, manual diaphragm valve, proportional solenoid valve

Burkert/Gemi/DK

GermanyChina

Standard fitted

Rotameter

Chengfeng

China

Standard fitted

Filter cartridge

Shanghai

Standard fitted

Pressure sensor

Burkert

Germany

Standard fitted

Part Name

Brand

Origin

Remark

pH electrode

Hamilton/Mettler

Switzerland

Standard fitted

DO electrode

Hamilton/Mettler

Switzerland

Standard fitted

pH signal transmission line

Hamilton/Mettler

Switzerland

Standard fitted

DO signal transmission line

Hamilton/Mettler

Switzerland

Standard fitted

touch screen

MCGS

Well-known domestic brands

Standard fitted

PLC

Siemens

Germany

Standard fitted

Thermostatic water pump

WILO

German brand

Standard fitted

Electric machine

SEW

Germany

Standard fitted

Frequency converter

BOSCH

Germany

Standard fitted

controlling software

Bioreactek™

China

Standard fitted

The electromagnetic valve

Sanlixin/Julang

Zhejiang/Shanghai

Standard fitted

Pneumatic diaphragm valve, pneumatic angle seat valve, manual diaphragm valve, proportional solenoid valve

Burkert/Gemi/DK

GermanyChina

Standard fitted

Rotameter

Chengfeng

China

Standard fitted

Filter cartridge

Shanghai

Standard fitted

Pressure sensor

Burkert

Germany

Standard fitted