Fluorescence Colony Analyzer

CA-FLuo2 Fluorescence Colony Analyzer is high-end flagship products designed for fluorescence analysis; it integrates the viable count, inhibition zone measurement, strain screening three functions into one. Three-color LED surround lighting, adjustable color temperature, so that the colonies image closer to that of  under natural light; dual wavelength UV meet disinfection, mutagenesis and fluorescence excitation needs; highly sensitive, large-array CCD makes Colonies image clearer, more suitable for obtaining fluorescence image, such as fluorescent colonies, fluorescent ring. FLuo2 equipped with 28 kinds of new algorithms, professionally designed strain screening module can be achieved: double ring analysis, inhibition zone measurement, the selection of specific bacteria, different strains identification.

Main Functions and Technical Index

1. Illumination system

1.1 Light source

1. Visible light: Highlight the tri-color LED structured light
2. 254nm UV: for cavity disinfection, UV mutagenesis
3. 366nm UV: excitation Escherichia coli, Intestinal flora fluorescent, green fluorescent protein

1.2.Light path and illumination control

1. Closed black box: the elimination of stray ambient light interference
2. Upper light source: episodic 360 ° flexible shadowless lighting
3. Lower light source: floating crystal sharp dark-field illumination
4. Upper light ,lower light, double light, ultraviolet, free to switch
5. Color temperature adjustable(3500K-8500K), light intensity adjustable

2.Digital imaging

1. HD industrial fixed lens: 8mm, 5.0 mega-pixel, 2/3 ", Distortion <-1%, C-Mount
2. Normal temperature sensitivity CCD camera: 2/3 "color SONY CCD sensor, 1.45 Mega Pixels, C-Mount

3.Colony Analysis Module

3.1. Basic colony counting function

1. Type plate: pouring, coating, membrane filtration, spiral plate, 3M paper, perforated plate
2. One key Intelligent Counting (6 mode): Large colonies ,tiny colonies, gray colonies, spreading colonies, certain colonies, multicolor mixed bacteria
3. Whole dish colony statistics: total number of colonies statistics, classified  display according to 25 gear sizes.
4. Selected area statistics: can do statistics with alternative circular, rectangular, arbitrary delineation of regional.
5. Diameter classification statistics: set diameter range, statistics specific colony size
6. Mouse click statistics: Fast tag, add colonies, suitable for counting colonies at the dish edge
7. Colony adhesion segmentation: automatic segmentation colonies adherent to each other, chain colonies can be splitted or not determined by the users.

3.2. Advanced colonies statistics

1. Spiral colonies statistics: automatic counting spiral tablet according to FDA standards, support index mode, slow index mode, homogeneous mode ,proportion mode, lawn mode. Compatible with spiral plater of United States SBI, Spain IUL
2. Dynamic adjustment statistics: statistics can be dynamically adjusted and corrected, quick access to the best statistical results.
3. Estimated deviation statistics: for colonies with many colors and  complex cases.
4. Level set multiple model algorithm: search operator, get the best image segmentation, adapt to background transformation of the culture medium
5. Specific colony statistics: According to the colony color, size, contour feature, identify specific colonies
6. Trans statistics: for colony type is extremely complex and medium uniform background
7. High adhesion bacteria statistics: for division calculation of multiple adhesion bacteria

3.3. The mesh filter and 3M test piece

1. One key statistics for black solid line grid
2. 3M total bacteria test piece, 3M S. aureus test piece: one key statistics
3. 3M coliform test piece, 3M E. coli / coliform rapid test piece: one key statistics + manual choose

3.4. Typical bacteria filter

1. Bacteria, impurities removed: According to distinguish shape, size, color, automatically remove bacteria, impurities.
2. Color classification statistics: according to color accuracy, the proliferation , colony size, contour feature, specific screen colonies
3. Multicolor automatic clustering: clustering based on color accuracy, automatically distinguish between 24 kinds of different colors of the colonies
4. Specifies multicolor filter: filter 1-8 kinds of colors specified colony at one time
5. Transparent Circle Characteristics analysis: Suitable for inhibition zone, hydrolysis ring, color ring, dissolved calcium circle, hemolysis ring, drain ring, dissolved phosphorus loop analysis
6. Bi-color ring auto filter

3.5.Description of colonies characterization

1. Bacteria, yeast colonies: color, size, shape, surface morphology, edges, gloss, transparency and other features, intelligent descriptions and ordering
2. Fungi, actinomycetes colonies: the front color, negative color, size, surface morphology, edge, texture, and other characteristics of intelligent descriptions and ordering

3.6. Microbial Limit Analysis Tools

1. Check the applicability of the medium
2. Control bacteria examination - colony morphology

3.7. Specific Analysis

1. Mold detection: quantitative analysis mildew Ratings
2. Series statistics: for complex colonies with uneven medium background
3. Parallel statistics: suitable for porous plate, OPKA, SBA analysis

3.8. Advanced tools

1. Clear grid: the elimination of interference of filter grid background
2. Manual counting correction: add or delete colonies
3. Decontamination areas: the mouse to outline any contaminated areas, automatically remove colonies amount of the contaminated areas
4. Eliminate background text: automatically eliminate marker interference
5. Background markings removed: automatic elimination of dish stains interference
6. Artificial adhesion segmentation: manual division multiple adhesions colonies
7. Parameters automatic conversion: diameter of dish, sample dilution input, automatic conversion
8. Text, graphics annotation: all kinds of drawing tools and text embedded in English and Chinese

3.9. Calibration and Measurement

1. Instrument calibration: The instrument equips with calibration, manual correction calibration
2. One-touch rapid measurement: one key measure a large colony ,suitable for single colony analysis of fungi, actinomycetes
3. Full dish automatic measurement :whole dish colonies equivalent diameter, area, long and short   diameter, perimeter, roundness analysis
4. Accurate manual measurement: length, angle, curvature, area, arc, arbitrary curves

4. Inhibition Zone Analysis Module

4.1. Szone inhibition zone multi-mode measuring technology

1. Automatic detection: accurate edge detection based on inhibition zone profile, suitable for sharp edges, circular inhibition zone
2. Quasidisks approximation: Approximation based on a circular zone of inhibition profile ,fitting for edge cracking, non-circular zone of inhibition
3. Manual inspection: click the mouse on the edge of the zone of inhibition by three times into round  ,suitable for the fuzzy edge of the zone of inhibition

4.2. Determination of the potency of antibiotics

1. A dosage titer assay method: for USP
2. Two doses, three doses of law and Consolidate: For Chinese Pharmacopoeia 2010 Edition
3. Repeatability self-test: relative error ≤0.01%, repeated accuracy ≤0.002mm
4. Homogeneity self-test: relative error ≤0.05%
5. Measure difference between platform ≤0.2%

4.3. sulbactam β- lactamase test

1. Water verification: According to (A), (B), (D) produced inhibition zone, D-C ≧ 3, B-A ≦ 3, the determined system is established.
2. Automatically detects three parallel samples (A), (B), (C), (D) inhibition zone, and data import
3. Automatically calculate the average of parallel test, smart discrimination the positive and negative  characteristic of the results.
4. Automatic alarm for invalid report

5.Database and Image Processing Module

5.1. Image Processing

1. Image adjustment: grayscale, negative phase diagram conversion; brightness, contrast, saturation adjustment; RGB adjustment
2. Image enhancement: sharpen, enhance adaptive
3. Image filtering: medium filter, high pass filter, a Gaussian filter, low pass filtering, queue filtering, high-pass Gaussian
4. Edge detection: Sobel operator, Robert operator, Laplace operator, the vertical detection, horizontal detection
5. Morphological operations: erosion, dilation, opening operation, closing operation

5.2. Database

1. Data storage, intelligent query
2. Data output: output statistics to Excel table

Data security: operator permissions, data modification permission settings

6. Specifications and configuration

1. CA2 Fluo fluorescent colonies analyzer host one unit
2. colonies analysis software, automatic inhibition zone measurement software, software for measuring the potency of antibiotics, sulbactam β- lactamase test software
3. advanced integrated computer